Abstract:

Objective

To detect the expression of urokinase-type plasminogen activator (uPA), matrix metalloproteinase-26 (MMP-26)in breast invasive ductal carcinoma(IDC) tissue, measure the microvessel density (MVD), investigate their correlation with clinical pathological parameters of IDC,the correlation of uPA and MMP-26 expression and the relationship with MVD.

Methods

Immunohistochemical SP method was applied to detect the expressions of uPA and MMP-26 in 85 cases of breast IDC (observation group) and 30 cases of breast fibroadenoma (control group). MVD was marked by CD105. Using SPSS17.0 statistical software, the relationship between clinical parameters and the protein expression of uPA,MMP-26 were analyzed by chi-square test. Because MVD had non-normal distribution and heterogeneity of variance, the sample means of different groups were analyzed by nonparametric test. Protein expression were analyzed using Spearman rank correlation test.

Results

(1)The positive rates of uPA and MMP-26 were 62.4%(53/85),64.7%(55/85) in observation group, and 26.7% (8/30), 16.7% (5/30)in control group, respectively(all P＜0.05). (2) In IDC tissues, the correlation was statistically significant between uPA expression and lymph node metastasis,histological grading,pTNM staging (all P＜0.05). The correlation was statistically significant between MMP-26 expression and lymph node metastasis,histological grading, and estrogen receptor (all P ＜0.05). uPA expression had a positive correlation with MMP-26 expression(r=0.341,P=0.002).(3)The positive rates of uPA and MMP-26 in tissue with high MVD were significantly higher than those in tissue with low MVD(P＜0.05).

Conclusion

uPA and MMP-26 are over-expressed in IDC tissue, which can promote tumor angiogenesis.

Key words: breast invasive ductal carcinoma, uPA, matrix metalloprotainase 26, microvessel density, angiogenesis